Vol. 10, Issue 12 (2021)

Author(s):
Yashwant Raut, ER Vaidya and Pallavi Sasane

Abstract:
In order to study the effect of mutagenesis on germination and growth in sesame, the seeds of two varieties ‘AKT-101 and PKV-NT-11’ were irradiated with 4 gamma radiation doses (viz., 400Gy, 500Gy, 600Gy and 700Gy) at BARC, Mumbai. About 50 seeds of each dose along with control in five replications were placed for germination in laboratory and observations were recorded on percent germination, root length (cm), shoot length (cm). Besides, about 100 treated seeds per dose were grown in randomized block design with five replications at research field of agricultural botany, Dr. PDKV, Akola and observations on percent germination, plant survival, and reduction in plant survival over control were recorded. In laboratory condition, the germination (%), shoot length and root length decreased progressively with increasing doses of gamma rays in both varieties. LD₅₀ was observed between 500 and 600 Gy in both varieties. Under field conditions, the highest survival reduction over control in both the varieties was recorded in 700Gy (84.41 in AKT-101 and 86.40 in PKV-NT-11). The maximum percent survival of M₁ plants in variety AKT-101 was observed in 500Gy dose (64.63%) and for variety PKV-NT-11, it was 400Gy dose (68.67%) respectively. Higher doses of gamma radiations caused considerable reduction in all parameters.