Vol. 10, Issue 12 (2021)

Author(s):
PN Jagadev, Sashikala Beura and HN Patro

Abstract:
The present investigation was undertaken to study the extent of genetic diversity present among 15 genotypes of turmeric (Curcuma longa L.) collected from High Altitude Research Station, Pottangi, Koraput representing a wide range of geographic locations of India using the RAPD and ISSR markers. A total of 6 RAPD and one ISSR markers were amplified and scored for bands. Data analysis was done using NTSYS-pc version 2.0 software and dendrograms were constructed following UPGMA and bootstrap analyses. The primers resulted in the amplification of 65 loci and out of these, 64 bands (98.5%) were polymorphic. Among the polymorphic bands, 8 were found to be cultivar specific. Highest resolving power and RAPD primer index was found for the primer OPN 16. Maximum similarity (0.75) was found between cvs. PTS 43 and PTS 51 and cv. RH 5 was clearly distinguished from rest of the cultivars. The genetic variations existed among the cultivars as revealed by RAPD and ISSR analyses might expedite the crop improvement in turmeric.