Vol. 10, Issue 12 (2021)

Author(s):
SB Tele, RM Khadtare, CT Kumbhar, SN Hasbnis and NA Napte

Abstract:
Present study revealed that all six bio-agents evaluated and exhibited antifungal activity against test pathogen (Alternaria alternata) and significantly inhibited mycelial growth over untreated control. Out of six bio-agents, Trichoderma viride showed least mycelial growth of test pathogen (15.70mm) and highest mycelial growth inhibition (77.57%). T. harzianum had 31.20 mm of mycelial growth and a growth inhibition of 55.42 %, which was at par to T. hamatum, which had 31.80 mm of mycelial growth and a growth inhibition of 54.57 %. The bioagent Pseudomonas fluorescence was shown to be the least efficient, with 54.30 mm of mycelial development and a 22.42 % inhibition of the test pathogen. Nine different fungicides at different concentration were evaluated in vitro by poisoned food technique. Among these nine fungicides cent per cent growth inhibition of A. alternata was recorded in Propiconazole 25% EC @ 0.1 % concentration tested. The next best in order of efficacy was Azoxystrobin 11% + Tebuconazole 18.3% w/w SC @ 0.1% followed by Difinoconazole 25% EC @ 0.05% and Carbendazim 12% + Mancozeb 63% WP @ 0.25 % which reported 88.12%, 84.03% and 80.00% growth inhibition respectively. Tebuconazole + Trifluoxystrobin 25% WG @ 0.06 % showed 77.77% inhibition which was at par with Tebuconazole 25.9% EC @ 0.1 % which was reported 76.03% growth inhibition. The fungicides viz., Propineb 75% WP @ 0.25%, Mancozeb 75% WP @ 0.25% were resulted in 74.08%, and 70.03% inhibition of A. alternata respectively. While, Thiophanate methyl 70% WP @ 0.05% was observed to be least effective in per cent growth inhibition of A. alternata as compared to other fungicides at their respective concentrations tried in vitro.