Vol. 10, Issue 12 (2021)

Author(s):
Shalaka Ahale, Gaurav Rakhonde and Sheenam Bhateja

Abstract:
Disease diagnosis is the first step in the management of plant diseases. Sustainable management of plant diseases includes efficient diagnostics for surveillance, detection and identification including the development and strategic deployment of resistant varieties. Nanopore sequencing is newly introduced platform which mainly work on sequencing of nucleic acid. An Oxford Nanopore technology (ONT) is a third generation platform used for sequencing of native nucleic acid strands, which is especially well suited to the production of high-quality assemblies of highly repetitive plant genomes. A motor protein was employed to shuttle single-stranded DNA or RNA through a nanopore in Nanopore sequencing device to generate nucleotide dependent electronic current signals which enables the analysis of pathogenic nucleotides. Despite of having several advantages like long read capacity, cost effectiveness and real time analysis, the current scenario of various companies is shifting towards the betterment of the accuracy results by working on the commercially available nanopore.