Vol. 11, Special Issue 2 (2022)

Author(s):
Megha SA, Rajesh A, Chalam MSV and Mohan Naidu G

Abstract:
Molecular characterisation of the Callosobruchus maculatus (Fabricius) populations collected from major pulse growing districts of Andhra Pradesh viz., Chittoor, Kurnool, Guntur, East Godvari and Srikakulam was carried out at Department of Entomology, Institute of Frontier Technology, RARS, Tirupati during 2020-21by using partial mitochondrial cytochrome B (CytB) primer. The nucleotide sequence obtained were blasted in NCBI database and they were confirmed as C. maculatus with 98 per cent similarity. Intra specific variability of C. maculatus sequences assessed through multiple sequence alignment revealed that there were no nucleotide variations in the nucleotide sequences of the populations. The present study confirmed the genetic identity of C. maculatus populations collected from five surveyed location in Andhra Pradesh.