Vol. 11, Special Issue 3 (2022)

Author(s):
Thota Joseph Raju, SN Vasudevan, Basavae Gowda, SR Doddagoudar, TC Suma, B Kisan and RV Beladhadi

Abstract:
The objective of this research was to identify SSR markers associated with hard seediness (seed coat permeability) and electrolyte leaching in soybean genotypes. The polymorphism survey was carried out using five SSR markers, of which three were polymorphic. These markers were used for genotypic analysis of 20 soybean genotypes. To assess the hard seediness (seed coat permeability) and electrolyte leaching, seeds of 20 genotypes are selected phenotypically from 96 genotypes which were subjected to seed coat characterstics screening in laboratory. A total of three (Satt 371, Satt 281and Satt 434) independent SSR markers were significantly associated with hard seediness (seed coat permeability) and electrolyte leaching. Markers Satt 371, Satt 281and Satt 434 produced specific allelic bands with respect to seed coat permeability, testa colour and electrolyte leaching. Satt 371, Satt 281and Satt 434 produced allelic bands of 180 bp, 220 bp, 235 bp respectively for minimum seed coat permeability and electrolyte leaching, specific for black and brown colour testa soybean seeds. Satt 371, Satt 281and Satt 513 produced allelic bands of 120 bp, 200 bp, 183 bp respectively for maximum seed coat permeability and electrolyte leaching, specific for yellow colour testa soybean seeds.