Vol. 11, Special Issue 3 (2022)

Author(s):
A Hafiz, DN Kalita and M Islam

Abstract:
A study was undertaken to determine resistance against albendazole (ALB), fenbendazole (FBZ) and closantel (CLS) in gastrointestinal nematodes of goat in an organized farm of Assam. The resistance was first investigated using faecal egg count reduction test (FECRT) in naturally infected goat which was further confirmed by in vitro egg hatch assay (EHA). Pre- and post-treatment copro-culture was performed to identify the species and genera of nematodes. In an organized goat farm of Assam the anthelmintics like albendazole, fenbendazole and closantel were found to be resistant i.e. the percent efficacy was not observed more than 95% of these three anthelmintics. The ED₅₀ value for fenbendazole, albendazole and closantel was recorded as 0.3506 mg/ml, 0.3357 mg/ml and 0.2330 mg/ml, respectively. The relative potency of closantel with respect to fenbendazole was found to be 66.46 per cent indicates small amount of closantel is sufficient to 0.3506 amount of fenbendazole. In vitro egg hatch assay after logistic fit of response using fenbendazole, albendazole and closantel were estimated. The results of the survey indicated multiple resistance in Haemonchus contortus and Trichostrongylus spp. to benzimidazoles and closantel in this farm.