Vol. 11, Special Issue 4 (2022)

Author(s):
Shivangi Sharma, Devendra Gupta, Amita Tiwari, Kabita Roy, Ajit Pratap Singh, RK Sharma and Pankaj Asati

Abstract:
The PCR assays are a boost to new developments in diagnostics especially for Leptospirosis which is fastidious organism and other diagnosis techniques such as culture and MAT are time consuming. The present study aimed at an early diagnosis of disease to move forward with treatment, thus PCR proved to be an effective tool. A total of 400 cattle were screened under the study. The cattle belonged to the organized dairy farms of Jabalpur. Out of 400 cattle, 70 blood samples were collected with a history of repeat breeding, abortion, hemogalactia and mastitis. Polymerase chain reaction was performed for early diagnosis of disease under the study in serum samples of suspected cattle. 18 out of 70 (4.5%) samples were found positive for leptospirosis with G1/G2 primers.