Vol. 11, Special Issue 4 (2022)

Author(s):
M Asokkumar, M Elaiyaraja, T Devi and A Ramesh

Abstract:
Rabies virus is a neuro tropic which can be cultivated in a wide variety of host cells like primary embryo fibroblast, MRC – 5 and Vero cells using either traditional or different types of bioreactor system for bulk antigen production. Such cultivation is extremely important to obtain the knowledge about virus itself as well as for producing large quantities of virus for the production of vaccine. The production of high yield of virus depends on the factor like quality of cells, media used and multiplicity of infection (MOI). High yield of virus can be obtained when cells in suspension are infected and then allowed to form monolayer. The present study was carried out in Vero Cells from passage 141 to passage 147 using rabies virus production methods as concurrent validation for ensuring the consistency of rabies virus yield in Vero cells. Viral Harvest yielded at different passage level of Vero Cells was subjected to virus titration by fluorescent antibody technique (FAT). The analysis of result showed that the virus yield is consistent at passage level of Vero cells from 141 to 147 and all viral harvest titre were well above the set limit of log₁₀⁵ CCID₅₀/mL. Hence it is demonstrated that consistent production can be obtained with Vero cells at Passage level at the beginning (P₁₄₁) and end (P₁₄₇) of the intended span of use without having any problem of residual substrate DNA in the final vaccine formulation.