Vol. 11, Special Issue 4 (2022)

Author(s):
Monu Karki, Dr. KK Rajak, Praveen Singh, Arfa Fayaz, Kiran, Ajay Kumar Yadav, Mukesh Bhatt, Vishal Rai, Chris Einstein and RP Singh

Abstract:
Canine distemper (CD) is a highly contagious and invariably fatal disease of domestic dogs and other carnivores having the second highest mortality rate in dogs, after rabies. The number of CDV outbreaks in vaccinated dogs and wildlife has increased in past, which demands the effective disease control strategy. The present study was designed to assess the potential of antibody detection in competitive mode by the LFA. Prior to testing serum samples, they are incubated with a fixed quantity of CDV antigen. The complete neutralization of antigen by the presence of antibody in the serum sample results in no binding at the test line. The intensity of the red band in the test line is inversely proportional to the concentration of antibodies in serum samples. The titre values of serum samples were primarily determined by virus neutralization test (VNT). Based on its titre values, the serum samples having titre up to 1:32 were found to be detected by LFA. The related viruses like PPRV, Measles, Canine Adenovirus, and Parvovirus serum samples did not show any cross-reactivity. Therefore, the optimized mAb-based LFA can be used as an on-site diagnostic tool for antibody detection in dogs as well as wildlife species.