Vol. 11, Special Issue 5 (2022)

Author(s):
SK Saranya, Marykutty Thomas, R Thirupathy Venkatachalapathy, TV Aravindakshan and K Raji

Abstract:
The single nucleotide polymorphisms (SNP) in gonadotropin releasing hormone receptor (GNRHR) gene contributes substantially to the litter size trait in goats. In the present study, analytical validity of tetra primer amplification refractory mutation system polymerase chain reaction (tetra primer ARMS PCR) assay and PCR restriction fragment length polymorphism (PCR-RFLP) were compared based on sequencing method results for genotyping of GNRHR promoter SNP (rs642351318; A/G) in goats. The sensitivity and specificity were 100 per cent for tetra primer PCR method, sensitivity and specificity were 100 and 97.43 percent respectively for PCR-RFLP. The results indicate that tetra-primer ARMS PCR provides an easy and cost-effective technique for GNRHR SNP genotyping.