Vol. 11, Special Issue 6 (2022)

Author(s):
M Elanchezhian, K Brindha, Sathaiah Gunaseelan and U Dhanalakshmi

Abstract:
The quality of sperms in any semen sample plays an important role in ART irrespective of any procedure that is carried out. Various parameters are considered in evaluating a semen sample to assess its fertility potential before any ART procedure is performed. Basic criteria like the concentration of the sperms, motility, and morphology are usually assessed when semen is used in artificial insemination to increase the success percentage in animals. The same protocol is followed in humans to evaluate the male fertility. Despite this analysis, there are other semen factors involved in determining the male fertility potential. Factors such as viability, acrosomal integrity, DNA fragmentation in sperms, etc., also affect the fertility potential of sperms which in turn affects the embryo quality and ART outcomes. Hence it is mandatory to study those factors to get a clear picture on the male fertility. It has also become a necessity to treat those cases to improve the quality of the sperms to avoid various challenges such as poor embryonic development, implantation failures, miscarriages, etc., The aim of this study was to find out whether exogenous supplementation of the anti- oxidant Coenzyme Q10 to sperm wash medium which is used to process the raw semen has any positive effects on the quality of sperms post preparation. Bull semen samples were processed using sperm wash medium and sperm wash medium fortified with CoQ10. The semen parameters of the post wash samples were observed and studied. There were observable significant differences found between post wash samples processed using sperm wash medium and sperm wash medium fortifed with CoQ10. Exogenous supplementation of CoQ10 to sperm wash medium had positive effects on semen parameters.