Vol. 11, Special Issue 6 (2022)

Author(s):
Lalit Kumar Saini, Pavan Kumar Mittal, GS Gottam, Barkha Gupta and DR Bilochi

Abstract:
Present study was designed to investigate the changes in physiological and haematological attributes during different seasons in Murrah buffaloes. This study revealed a substantial seasonal impact on all physiological and haematological attributes. Physiological indicators such as rectal temperature, respiration rate and pulse rate had greater values in the hot-humid season than winter season. Physiological attributes were recorded prior to blood sample collection during both season. The rectal temperature (RT), respiration rate (RR) and pulse rate (PR) was measured with electronic thermometer inserting into rectum, through nostrils during normal respiration and through a point on the underside of the base of the tail (middle coccygeal artery), respectively. Blood samples were collected aseptically from jugular vein using 18 gauge needles in blood vacutainer BS EDTA K3 vial (2ml) and then mix each blood sample. The haematological attributes were determined by Spincell 5 Compact Vet Mode Automated Blood Analyzer. The mean±SE values of Total Erythrocyte Count /RBC count, Neutrophil (%) showed a significantly (P≤0.01) higher level during hot-humid than winter season while Lymphocyte (%) was significantly (P≤0.01) higher in winter as compared to hot-humid season. The mean±SE values of Total leukocyte count/WBC count were significantly (P≤0.05) elevated during winter than hot-humid season whereas a non-significant (P>0.05) effect of hot-humid and winter season was observed on the mean±SE values of Monocyte (%), Eosinophil (%), Basophil (%). This study showed effect of winter and hot-humid seasons on the health of Murrah buffalo in terms of variation in different physiological and haematological attributes which are important indicators of health status in animals.