Vol. 11, Special Issue 6 (2022)

Author(s):
Kapale PM, AS Nagvekar, PM Kekan, BN Ramteke, NR Dagali, SD Ingole and GM Gadegaonkar

Abstract:
The present study was conducted on Holstein Friesian x Gir crossbred (n=8) bulls. The semen samples were collected throughout the year using an artificial vagina at one-month intervals in three seasons (winter, summer, and monsoon). The semen samples collected from the bulls were divided into two parts: one was used for analysis as fresh semen sample, and the second was diluted using an egg yolk extender and cryopreserved for future use. Seminal plasma enzymes like ALP, ACP, CPK activity, and seminal plasma testosterone in fresh semen samples during different seasons were statistically significant (P<0.05). In diluted (pre-freeze) seminal plasma, ALP and CPK activity showed statistically significant differences between seasons. Whereas, GGT in fresh and diluted semen showed a non-significant difference. In frozen (post-thaw) semen samples, the differences in the values of ALP, LDH, CPK and GGT enzyme activities showed significant differences (P<0.05).