Vol. 11, Special Issue 6 (2022)

Author(s):
Bithika Halder, Samar Sarkar, Samiran Bandapadhyay, Prasanta Kumar Sarkar, Amit Raj Gupta and Subhasish Batabyal

Abstract:
This study look for the efficacy of selective ayurvedic nanoparticle drug against bacterial infection in murine model.As the antimicrobials are rapidly losing their efficacy, it is urgent to further our research for new and effective alternative to combat such multi-drug resistant isolates. Medicine consists nanoparticles known as nanomedicine which desires deliver research tools and clinically reformative devices in the near future for having many advantages such as specific drug delivery system, reduced toxic effects while continuing therapeutic effects, biocompatible, faster and safe medicine. Nanoparticle drug containing particles ranges from 1 to 100 nm in size, an application of nanotechnology which made its debut with greatly increased possibilities in the field of medicine. In the present study ayurvedic herbo-metallic drug which is reported as nanoparticle drug, Rasamanikya was evaluated against the ESBL producing/carbapenem resistant E. coli bacterial infection in mice model. Rasamanikya is used in powder form at 16.25 mg/kg dose in mice. One microlitre of bacteria (corresponding to 1 ×10⁷ or 1 × 10⁸ CFU for immunocompetent mice and 1 ×10³ or 1 × 10⁵ CFU for immunocompromised mice) with normal saline solution was inoculated subcutaneously to produce infection. On the next day skin lesion was developed in the inoculated site and the mice was treated with Rasamanikya @ 16.25 mg/kg dose orally twice daily for 7 days with vehicle (honey). The drug was also used for antiseptic therapy on the lesion by mixing with soft paraffin in 1:1 ratio twice daily. After 7 days of the treatment the lesion was almost healed and the mice was recovered. For haemato-biochemical test blood sample was collected by heart puncturing method and tissue sample were collected from the mice to investigate the histopathological changes after sacrificing of animal by cervical dislocation.