Vol. 11, Special Issue 6 (2022)

Author(s):
Mandeep Singh Azad, Dibyendu Chakraborty, Kawardeep Kour, Simran Singh, D Kumar, Nishant Kumar, Aditi Lal Koul and Vikas Khajuria

Abstract:
Apoptosis of programmed cell death is an important phenomenon of body immune response. Inhibitor of Apoptosis gene (IAP-1) is involved in different biological activities like binding and inhibiting caspases, regulating cell cycle progression, and modulating receptor-mediated signal transduction. The present study was undertaken with the main objectives of molecular characterization of IAP-1 gene in local native chicken of Poonch region. RNA Purification Kit (HiPura) was use for RNA extraction from Poonchi blood. After cDNA synthesis, IAP-1 gene of 544 bp size was amplified from cDNA using specific primers designed by Primer 3 software. Direct sequencing was carried out by Sanger sequencing with both forward and reverse primers to obtain 544 bp sequence product. The sequences were studied by using BioEdit and MEGA X softwares. The sequence results of IAP-I gene of Poonchi chicken and Kadaknath chicken were compared with, Leghorn, Fayoumi breed of chicken and also with other sequence of Gallus gallus complete Cds, Centrocercus urophasianus, Mus musculus, Gallus Gallus partial Cds, Rattus norvegicus, Tympanuchus phasianellus. AT content were higher then GC content for IAP-1 gene. Sequence results were analyzed for synonymous (S) and non-synonymous (NS) changes. No SNP variation within Poonchi population as well as with Kadaknath population were observed. Fayoumi breed showed 2 (NS) changes. Highest number of NS changes were observed with Mus musculus. The Genetic distance result showed no dissimilarity between Poonchi chicken and Kadaknath breed. Highest genetic distance was observed with Tympanuchus phasianellus.