Vol. 11, Special Issue 7 (2022)

Author(s):
Hemlata Chouhan, Urmila Pannu and Manju Nehra

Abstract:
Total 160 lactating animals of Rathi cattle were selected to analyze for genetic variation in STAT5A gene. DNA was isolated from blood samples by kit method. Species- specific primers were constructed for exon-7, intron 9–10 and intron 15–exon 16 of STAT5A gene based on available sequences of STAT5A gene in the NCBI GenBank database. a polymerase chain reaction in a final reaction volume of 25μl were prepared for regions of STAT5A gene and after amplification fragments of 215, 224 and 379 bp of exon-7, intron 9–10 and intron 15–exon 16 region of STAT5A gene obtained, respectively. Genetic variation of all three regions of STAT5A gene was analyzed by SSCP method. In exon-7, two alleles (A, B) and three genotypic patterns (AA, AB and BB) were observed with 0.738, 0.262, 0.594, 0.288 and 0.118 frequencies, respectively. In intron 9–10, polymorphism was observed with two alleles and two genotypic patterns with 0.921, 0.069, 0.843 and 0.156 frequencies, respectively. In intron 15–exon 16 region of STAT5A gene polymorphism was observed by SSCP method in studied population with 0.937, 0.062 0.875 and 0.125 frequencies of two alleles (A, B) and two genotypic patterns, respectively. In studied Rathi cattle population polymorphism was observed in STAT5A gene. This can be useful in study of milk production traits in Rathi cattle.