Vol. 11, Special Issue 7 (2022)

Author(s):
Hemlata Chouhan, P Bano, R Mohta, B Gujar, U Pannu and M Mehra

Abstract:
The present study was undertaken to explore the genetic variability in part of intron-5 exon-5 portion of intron-6 region of OPN gene in 160 Rathi cattle. From each animal in selected population 2 ml blood were collected for DNA isolation by kit method. Species-specific primer was constructed based on available sequences of OPN gene in the NCBI GenBank database which used to amplify this region of OPN gene. A polymerase chain reaction in a final reaction volume of 25μl were prepared for region of OPN gene and after amplification fragments of 826 bp of part of intron-5 exon-5 portion of intron-6 region of OPN gene was obtained. Single-strand conformation polymorphism (SSCP) method was carried out to detect the genetic variation. Monomorphism was observed in selected region of the OPN gene. In this study this region of OPN gene not showed genetic polymorphism. Some studies shows polymorphism in this region of OPN gene. In this study presence of a single SSCP pattern was observed which indicates more commen pattern of this region of OPN gene. Further analysis of this gene in Rathi cattle is required to detect polymorphism and its association with milk production traits. Its variants would be used in the marker-assisted selection for the improvement of dairy traits in cattle.