Vol. 11, Special Issue 7 (2022)

Author(s):
S Tamizharasan, S Vairamuthu, N Pazhanivel, S Savitha, M Chandrasekar, KG Tirumurugaan and Ganne Venkata Sudhakar Rao

Abstract:
A five-year-old male, Labrador retriever dog weighed 35kg was presented to the Madras Veterinary College Teaching Hospital with the history of swelling on face, neck, leg and abdominal region. Clinical examination revealed enlargement of all the peripheral lymph nodes. Ultrasonography of abdomen revealed enlargement of mesenteric lymph node and iliac lymph nodes. Splenic images showed reticular pattern or Swiss cheese like appearance. Anatomically, it was classified as multicentric lymphoma. Hematology was normal and in serum biochemistry, increased level of total and direct bilirubin indicative of hepatic insufficiency and corrected by giving heptatonic and fluid therapy for a week. Fine needle aspiration cytology (FNAC) showed presence of large lymphoblastic cells with multiple nucleoli and a few mott cells. FNAC sample in normal saline was analyzed using flowcytometry with CD21 and CD3 monoclonal antibodies for immunophenotyping which showed 69.35% of aspirated cells were tagged with CD21 marker which indicated B type lymphoma. It was in stage ‘IV substage ‘a’ based on physical, clinical and ultrasound examination. CHOP protocol was given and partial remission was observed after one week of treatment. Animal was died due to hepatic insufficiency. Overall survival time of the dog was 20 days. Post mortem examination revealed all the lymph nodes were enlarged and heart was rounded appearance. Histopathological examination revealed effacement of nodal structure, capsular thinning and compressed peripheral sinus. Neoplastic cells were filled the medullary cord and compressed sinuses. Histologically it was classified as diffuse large B cell lymphoma. Metastasis was observed in liver, kidney, heart, lungs, brain and testis.