Vol. 11, Special Issue 7 (2022)

Author(s):
Renu, H Dadhich, M Mathur, PK Boyal, M Mehra, S Rani and Rajesh Mohta

Abstract:
Clostridium perfringens is a gram-positive, rods shaped, non-motile, and toxin-producing anaerobe responsible for producing several diseases in animals. It is widely spread in the environment and is normally found in the gastrointestinal tract of animals. The pathogenicity of this organism is associated with exotoxins.Clostridium perfringens is normally present in the intestine of animals and humans. The present study was conducted to molecular identification of the bacterial species specific gene from 16s rRNA with product size 481bp. Necropsy was performed on 362 sheep irrespective of age, sex and breeds. Out of these, 123 sheep suspected for enterotoxemia were processed for molecular confirmation of Clostridium perfringens from intestinal contents by using PCR. After conducting PCR, 66 sheep found positive for Clostridium perfringens.