Vol. 11, Special Issue 7 (2022)

Author(s):
Renu, H Dadhich, M Mathur, PK Boyal, M Mehra, S Rani and Prakash

Abstract:
Clostridium perfringens type D secrets epsilon toxin which causes enterotoxemia in sheep. Enterotoxemia can cause acute or superacute disease, with sudden death of the affected animal. It induces huge economic losses when large numbers of livestock are affected. The present study was conducted to molecular identification of the bacterial species specific and toxigenic gene from 16s rRNA with product size 481bp and epsilon (etx) with product size 655bp. A detailed necropsy was performed on 362 sheep irrespective of age, sex and breeds. Out of these, 123 sheep suspected for enterotoxemia were processed for molecular confirmation of Clostridium perfringens type D from intestinal contents by using PCR. After conducting PCR, 66 sheep found positive for Clostridium perfringens type D.