Vol. 11, Special Issue 7 (2022)

Author(s):
PM Sonkusale, RC Ghosh, Deveshkumar Giri, Poornima Gumasta, Neha Shukla, Vivek Kumar, DK Jolhe, SD Hirpurkar and Savita Bisen

Abstract:
Enteritis is one of important disease of chickens character­ized by diarrhoea, poor weight gain and in some flocks, high mortality. Chicken Parvovirus (ChPV) is one of the important viral agents involved in enteric disorders. The present study was aimed to detect the ChPV associated with enteritis in desi and kadaknath chickens of Chhattisgarh. Total of 151 desi and kadanath birds (1-18 weeks old) died due to enteritis with symptoms of diarrhea, vent pasting, ruffled feathers and poor growth were subjected to necropsy. Intestinal tissue samples collected from 151 birds were subjected to PCR assay for detection of ChPV by amplifying 561 bp sequence of NS gene. ChPV was detected in 31.78% (48/151) of intestinal samples collected chickens of Durg, Rajnandgaon, Balod and Raipur district of Chhattisgarh which suffered from enteritis. Lesions consisted of catarrhal enteritis, dilated, inflamed or pale intestinal wall with watery and gas filled contents, atrophy of immune organs and pancreas. Sequences of NS gene of ChPV showed 98.37%-99.19% nucleotide similarities with other previously published sequences. Phylogenetic analysis revealed that detected Indian isolate is grouped with Poland 1 and USA 2 isolates. The results indicated the presence of ChPV in Indian chicken flocks and its close association with enteritis.