Vol. 11, Special Issue 7 (2022)

Author(s):
Dinesh Kumar Saharan, Kavita Jaidiya, Yogendra Meena, Shivendra Kumar Bhalothia and Sunita Choudhary

Abstract:
The current study aimed to isolate and identify bacterial flora in milk of cattle affected with clinical mastitis. a total of 16 animals involving 23 quarters exhibiting clinical manifestations of mastitis in were included.Clinical mastitis was observed high in 4^th parity, early lactation stage and in hind quarters. On cultural examination of mastitic milk of 23 quarters of 16 cattle, 18 quarters (78.26%) were having single bacterial infection, whereas 5 quarters (21.73%) were having mixed infection. The bacteria isolated in this study were Staphylococcus aureus, accounting for 12 of the isolates (42.85%), followed by 6 (21.42%) E. coli, 5 (17.85%) Streptococcus agalactiae, 3 (10.71%) Klebsiella pneumoniae and 2 (7.14%) Streptococcus dysaglactiae, respectively. In mixed infection combination of four genera were found. Organisms isolated in mixed infection were Staphylococcus spp., Streptococcus spp., Klebsiella pneumoniae and E. coli. Major pathogens found were Staphylococcus spp., Streptococcus spp, Klebsiella pneumonia and E.coli on bacteriological culture.