Vol. 11, Special Issue 7 (2022)

Author(s):
P Karela, RK Dedar, A Chahar, RA Legha, M Suresha and N Kumar

Abstract:
The present study was taken to investigate cell viability assay for aqueous and ethanolic extract of Capparis decidua. Skin tissue was collected from horse and processed via sequential enzymatic tissue digestion and tissue explant method. Dermal fibroblast cells were maintained in DMEM culture medium supplemented with 10% FBS in a humidified incubator at 37 °C and 5 % CO₂ level. Aqueous and ethanolic extracts of these herbs were made through soxhlet apparatus and evaoporated by rotatory evaporative to get dried extract. MTT assay was used to evaluate noncytotoxic concentration of aqueous and ethanolic extracts of Capparis decidua. Aqueous extract of Capparis deciduas have shown more than 80% cell viability in all dilution concentrations. Ethanolic extract of Capparis has showed cytotoxicity up to 8 μg/ml concentration. So, it is concluded that Capparis deciduas have great therapeutic potential to treat variety of diseases because of active phytochemical that present abundantly in it.