Vol. 11, Special Issue 7 (2022)

Author(s):
M Fibi Rani, GS Sreenivasamurthy, M Udaya Kumar and P Kalyani

Abstract:
Trypanosomosis is a widely prevalent haemoprotozoan disease caused by a haemoflagellate, Trypanosoma evansi. The present study aimed to detect Trypanosoma evansi in dogs using microscopic methods like wet blood film examination and conventional staining techniques i.e., Giemsa staining, Field’s staining, Acridine orange staining at the field level. In the current study, 314 dog blood samples were collected and examined from different places in and around Hyderabad, Telangana, India. Out of 314 dogs, 5 (1.59%) dogs were found positive by Giemsa, Field’s, Acridine orange staining techniques and only 3 (0.95%) dogs were found positive by peripheral wet blood film examination for Trypanosoma evansi.