Vol. 11, Special Issue 7 (2022)

Author(s):
Nancy Jasrotia, MK Tandle, Anju Kujur, RG Bijurkar, Nagaraj, Ambika, MD Suranagi and Shrikant Kulkarni

Abstract:
The study was carried out to evaluate the effect of supplementation of almond oil (AO) and olive oil (OO) in Skim milk (SM) and TRIS egg yolk (TEY) extenders, on sperm quality parameters of the NARI Suwarna ram semen refrigerated for 72 h. Semen collected from five mature NARI-Suwarna rams was pooled and diluted to a final concentration of 0.8 x 10⁹ spermatozoa/ml in SM and TEY extenders supplemented with AO and OO at 0.25% v/v (SM, SMAO, SMOO, TEY, TEYAO, TEYOO). Semen was then stored at 5 °C and several sperm motility parameters were assessed by Computer assisted semen analysis (CASA) at 0, 24, 48 and 72h of storage. Randomized Block Design (RBD) was used to test the significant difference between extenders. Extenders of TEY (TEY, TEYAO and TEYOO) were found significantly (P<0.05) better as compared to SM, SMAO and SMOO. Addition of olive oil (0.25%) in TEY extender had beneficial effect in preserving rapid progressive sperm motility percentage better than in other extenders. Assessment of sperm motility parameters by CASA revealed TEY as better extender as compared to SM for preservation of refrigerated NARI Suwarna ram semen for 72 h.