Vol. 11, Special Issue 7 (2022)

Materials and Methods: A study was conducted to determine the antibiogram of bacterial species isolated from 2 female dogs diagnosed with pyometra. One sample was uterine fluid collected from one of the uterine horns using sterile needle and syringe in a sterile tube and other sample was a vaginal discharge collected by using sterile swab. The bacteria from both the samples were isolated, biochemical profiling was done and sensitivity towards antibiotics was determined by measuring the zone of inhibition on Muller Hinton agar.

Results: Staphylococcal species was isolated from both the samples. The antibiotic sensitivity of uterine fluid showed that lincomycin was the most sensitive antibiotic followed by enrofloxacin and gentamicin. Methicillin was most resistant antibiotic followed by kanamycin and ampicillin. While the vaginal discharge showed that cloxacillin was the most sensitive antibiotic followed by ceftriaxone and lincomycin, tetracycline was most resistant antibiotic followed by Ampicillin.

Conclusion: Lincomycin was found to be the most effective antibiotic against the bacterial species isolated from canine pyometra uterine fluid. While Cloxacillin was the most effective antibiotic against bacterial species isolated from vaginal discharge.