Vol. 11, Special Issue 8 (2022)

Author(s):
R Pradeep, A Senthilkumar and A Sangaran

Abstract:
Pheasants are susceptible to a wide range of diseases, in which enteropathogens are one of the major health problems, which might be transmitted from one group of birds to other. Seventy-six fresh dropping samples were collected aseptically using readymade transport medium swabs and transported immediately to the laboratory. All the samples were processed immediately for isolation and identification of important bacterial enteropathogen E. coli using standard conventional and molecular procedures. The isolation of E. coli began with collected cultural swabs were inoculated in EMB agar. KB010 is a comprehensive test system that can be used for identification biochemical characterization of E. coli. Molecular identification of E. coli by DNA extraction and polymerase chain reaction. Out of 76 samples, twenty three samples were positive (30.26% Per cent) and growth of E. coli organisms in EMB agar appeared as blackish centers with a green metallic sheen.Molecular Identification of E. coli by polymerase chain reaction the amplicon size was 477 bp.