Vol. 11, Special Issue 9 (2022)

Author(s):
CH Priyadarshini, P Eswara Prasad, K Aswani Kumar, D Nagalakshmi and D Madhuri

Abstract:
Fenugreek (Trigonella foenum-graecum) seeds (FS) are rich in phytochemicals, one of them is steroidal saponins, diosgenin. Diosgenin has several properties in treatment of various disorders, inflammation, hypercholesterolemia, diabetes, several types of infections, antioxidant effect, different types of cancer. It also has shown estrogenic effect. Due to its pharmacological and industrial importance, several extraction and analytical procedures have been developed and applied over the years to isolate, detect, and quantify diosgenin. One of the best methods in determining the quantity of diosgenin is by HPLC technique. In HPLC method, an isocratic binary system of acetonitrile/water (90:10 v/v), a flow rate of 1 ml/min with UV at 203 nm and a temperature of 33 °C, the retention time (RT) of standards of 10, 50 and 100 ppm diosgenin showed 24.94, 24.933 and 24.927min respectively while aqueous and methanol extracts of FS showed 24.927 and 25.033 min respectively. The calibration curve constructed based on three dilutions of diosgenin standard at concentrations produced a linear regression (r = 0.995) and the concentration of diosgenin in methanol and aqueous extracts were 5.233 X 10^-6 mg/ml and 151.649 X10^-6 mg/ml respectively.