Vol. 11, Special Issue 9 (2022)

Author(s):
Dr. Ravi Sikrodia, Dr. Daljeet Chhabra, Dr. Supriya Shukla, Dr. Hemant Mehta, Dr. SD Audarya, Dr. R Gangil, Dr. J Jogi and Dr. R Sharda

Abstract:
Livestock is an essential component of the Indian agricultural production system and is crucial to the advancement of the country. In dairy, calf diarrhoea is a complex disease that can have major financial and animal welfare ramifications. High risks of calf illness and mortality result in significant financial losses for dairy industries. The present study was undertaken to isolate Klebsiella from diarrheic cattle calves to assess the antimicrobial resistance pattern of these isolates by in vitro method. A total of 137 rectal swabs were collected from diarrheic calves directly from the rectum. BHI broth, MacConkey agar, EMB agar and Nutrient agar were used to isolate the Klebsiella. From 137 diarrheic samples of cattle calves, 13 (9.48%) Klebsiella isolates were isolated and identified on the basis of cultural, morphological, and biochemical characteristics. These Klebsiella isolates on subjecting to PCR using genus specific primers revealed the presence of an expected amplicon of 441 bp size (Plate 3) and, hence confirmed genotypically as Klebsiella. The in vitro AST revealed that all isolates of Klebsiella were 100% resistant to enrofloxacin followed by ampicillin (92.31%), and aztreonam, colistin and nitrofurantoin (84.62% each), ceftazidime plus clavulanic (76.92%), ceftriaxone and sulphadiazine (46.15% each), azithromycin (38.46%), and co-trimoxazole and tetracycline (30.77% each), and cefotaxime, ciprofloxacin, gentamycin and streptomycin (23.08% each). All the isolates were found to be multidrug resistant as each of the isolate was resistant to minimum one agent of at least three antimicrobial groups.