Vol. 11, Special Issue 9 (2022)

Author(s):
M Dhanushasree, A Thanga Hemavathy, D Malarvizhi and S Kavitha

Abstract:
Molecular diversity of fifty five pigeonpea genotypes were studied with fifteen microsatellite markers. Among them, four markers produced polymorphic bands and six markers were identified as monomorphic. The PIC value of these polymorphic markers ranged between 0.07 to 0.36. Marker CcM1251 (0.36) recorded the maximum PIC value and the minimum PIC value for the markers CcM0494 and CCac010 (0.07). Molecular tree constructed using Ordinal Neighbour Joining analysis grouped the fifty five genotypes into six major clusters. Among the six clusters, Cluster I was the largest with forty genotypes followed by cluster VIII (10), cluster VI (9), cluster II (5) and clusters V and VII (4). Cluster III and IV were the monogenic clusters with one genotype in each cluster. The study concludes that 38 percent of genotypes present in cluster I which signifies lack of divergence among the genotypes, while the genotypes viz., ICPL 11301 and CRG 16-07 were identified to be more diverse forms solitary cluster which can be utilized as parents for heterosis breeding programmes.