Vol. 11, Special Issue 9 (2022)

Author(s):
Sapna Tiwari and Pramod Mall

Abstract:
This study was conducted to investigate the effect of purification process on total phenol content of ethanolic extracts of propolis with respect to its geographical origin. Propolis samples collected from different geographical regions were extracted with 50% ethanol (at 1:30 w/v). Purification of ethanolic extracts was performed by centrifugation. Significantly higher total phenol was obtained in purified ethanolic extracts of propolis (200.565 mg gallic acid eq/g) than in impure ethanolic extracts (172.989 mg gallic acid eq/g). Moreover, propolis collected from regions of Pantnagar possessed higher total phenol than those collected from Haldwani region. Results of the current study indicated significant effect of interaction between geographical origin of propolis and purification process with respect to its total phenol content. Moreover, the process of purification induced improvement in propolis quality by escalating its total phenol content.