Vol. 11, Special Issue 9 (2022)

Author(s):
Ajit Kumar, SC Mandal, Surajit Baidya, Ruma Jas and Soumitra Pandit

Abstract:
Henna (Lawsonia inermis) has been used since antiquity to dye skin, hair and fingernails, as well as fabrics and it’s local name is ‘mehendi’. In this present study, henna leaves were used for preparation of aqueous and ethanolic henna leaves extract stains following standard protocol. Basing on clarity of staining, different gradations (+4, +3, +2, +1 & 0) were designed for extremely clear, clear, moderately clear, less clear and unclear, respectively for assessing four categories (aqueous extract with alum, ethanolic extract with, aqueous extract without alum, ethanolic extract .The data derived from the score values statistically (p>0.05) depicted equal clarity of the four categories of stains developed in the present study In this present experiment, herbal extract stain took less time (only 30 minutes) to give better staining quality of haemoprotozoa with good resolution whereas commercially available Giemsa stain generally takes 45 minutes. In the present study, henna leaves extract has got substantial worth to be used as eco-friendly, non-hazardous herbal stain in the field.