Vol. 11, Special Issue 9 (2022)

Author(s):
K Shravankumar, D Madhuri, M Lakshman and B Kalakumar

Abstract:
Glyphosate (GLP) mediated toxicity is due to excessive production of reactive oxygen species and ascorbic acid (AA) is a naturally occurring primary antioxidant which can scavenge or inhibit ROS production. A total of forty- eight (48) male rats were divided into four groups viz. group 1 served as control, group 2 served as GLP toxic control (500 mg/kg b.wt, orally), groups 3 and 4 rats were administered with AA (250 mg/kg b.wt, orally) and GLP (500 mg/kg b.wt) + AA (250 mg/kg b.wt) respectively for 21 days. Results showed significant (p<0.05) reduction in the body weights of rats treated with glyphosate alone. Hematological examination revealed a significant (p<0.05) decrease in the Total Erythrocyte Count (TEC), Total Leukocyte Count (TLC), Hemoglobin (Hb) concentration and Packed Cell Volume (PCV) whereas erythrocyte indices revealed a significant (p<0.05) increase in the Mean corpuscular volume and Mean corpuscular hemoglobin and in significant (p<0.05) decrease in Mean corpuscular hemoglobin concentration in rats treated with GLP alone. The serum biochemical assays revealed a significant (p<0.05) increase in the Aspartate Transaminase (AST), Alanine Transaminase (ALT), Alkaline Phosphatase (ALP), Total Proteins (TP), Blood Urea Nitrogen (BUN) and serum creatinine in GLP alone treated rats. A significant improvement in all the parameters was observed in rats treated with both ascorbic acid and GLP (group 4). The results concluded that supplementation of ascorbic acid ameliorates glyphosate induced hemato-biochemical alterations.