Vol. 11, Special Issue 10 (2022)

Author(s):
Ajit Kumar and SC Mandal

Abstract:
To develop eco-friendly herbal stains, Onion (Allium cepa) tunic were used and prepared aqueous and ethanolic Onion tunic extract stains by following standard protocol. Aqueous and ethanolic onion tunic extract stains showed good staining quality with appreciable contrast images of platyhelminthes parasites. Staining quality of aqueous and ethanolic onion tunic extract stains showed significant difference ((p<0.01) when compared with carmine stain and was found non-significant (p>0.05) when compared with different types of onion extract stains with each other for staining of platyhelminthes. Onion tunic extract herbal stain is eco-friendly, locally available and non-hazardous herbal extract stain could be a good substitute for commercially available hazardous and expensive conventional stains in the nearest future.