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Journal code(s)
- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
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Vol. 11, Special Issue 10 (2022)
Assessing the antidiabetic activity of peptides obtained from chicken intestine hydrolysate in canine pellet food using alpha amylase inhibitory assay
Author(s):
E Vimalraj, R Ramani, V Appa Rao, M Parthiban, R Narendra Babu, A Abinaya and S Arulkumar
E Vimalraj, R Ramani, V Appa Rao, M Parthiban, R Narendra Babu, A Abinaya and S Arulkumar
Abstract:
Chicken intestine was hydrolysed by protease P food grade fungal enzyme. Chicken intestinal hydrolysate was ultrafiltered using 10kda molecular weight cut-off and the filtrate is freeze dried. Then the low molecular weight peptides is incorporated in canine pellet feed at 5%, 6% and 7% levels. Canine pellet feed with different levels of incorporated peptides was assessed for antidiabetic activity using α-amylase inhibitory assay. The mean±SE values of IC50 value for anti-diabetic activity of bioactive peptide incorporated at different levels of 5%, 6% and 7% in canine pet food were 59.26±4.4 mg/ml, 364.28±13 mg/ml and 163.57±5.04 mg/ml respectively.
Chicken intestine was hydrolysed by protease P food grade fungal enzyme. Chicken intestinal hydrolysate was ultrafiltered using 10kda molecular weight cut-off and the filtrate is freeze dried. Then the low molecular weight peptides is incorporated in canine pellet feed at 5%, 6% and 7% levels. Canine pellet feed with different levels of incorporated peptides was assessed for antidiabetic activity using α-amylase inhibitory assay. The mean±SE values of IC50 value for anti-diabetic activity of bioactive peptide incorporated at different levels of 5%, 6% and 7% in canine pet food were 59.26±4.4 mg/ml, 364.28±13 mg/ml and 163.57±5.04 mg/ml respectively.
These values were then compared with the IC50 value for anti-diabetic activity of canine pet food prior to addition of bioactive peptides. Which was taken as control is 1313.85±52.8. The test of significance revealed that there was a highly significant difference between the different levels of bioactive peptides incorporated canine pet food. The 5% inclusion level was found to have less IC50 value of 59.26±4.4 µg/ml and better alpha amylase inhibition activity. The canine pet food with 5% incorporated bioactive peptides had lower IC50 value than the other levels indicating that the potency of the extracted peptides has been exhibited in the incorporated canine pet food.
Pages: 861-863 | 202 Views 104 Downloads
How to cite this article:
E Vimalraj, R Ramani, V Appa Rao, M Parthiban, R Narendra Babu, A Abinaya and S Arulkumar. Assessing the antidiabetic activity of peptides obtained from chicken intestine hydrolysate in canine pellet food using alpha amylase inhibitory assay. The Pharma Innovation Journal. 2022; 11(10S): 861-863.
Journal code(s)
- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
Main Menu
Special Issue
Copyright Form
Identifier
The Pharma Innovation Journal
