Vol. 11, Special Issue 10 (2022)

Author(s):
Shivani Mamane, N Jeyathilakan, Bhaskaran Ravi Latha, TMA Senthilkumar and P Raja

Abstract:
Schistosoma spindale is an obligate parasite of blood vascular system causing Bovine visceral schistosomiasis. It is economically important chronic disease characterized by hemorrhagic diarrhoea, weight loss, weakness, anemia and stunted growth in cattle and often goes under diagnosed. The present study was conducted to know the presence of Schistosoma spindale infection in Chennai, Tamil Nadu by morphology and molecular confirmation by PCR One hundred and eighty mesentery samples of cattle were collected from Perambur slaughter house, Chennai, Tamil Nadu, India during a period of October 2019 - March 2020 for screening visceral schistosomiasis.. Adult flukes were collected from 57 cattle by screening mesentery samples ranging from 1-808 / mesentery. Morphological identification was further confirmed by PCR by targeting the 16S rRNA gene. Upon gel documentation amplicon of size 330 bp specific for Schistosoma spindale was observed and confirmed by sequencing studies. Phylogenetic analysis revealed existence of commom ancestor from S. spindale of Thailand and Sri Lankan isolate.