Vol. 11, Special Issue 10 (2022)

Author(s):
Ramesh Kankanawadi, Basavaraj Awati, Ambadas and Shakuntala Kage

Abstract:
The present study was designed to study the molecular characterization of the Salmonella spp. from pigs. A total of 175 fecal samples collected from different pig farms which are located in Bidar, Hassan and Tumakuru districts of Karnataka. Based on the high specificity of primers targeting invar gene, they were employed for the specific confirmation of the Salmonella spp. isolates to genus level. All the Salmonella spp. suspected cultures subjected to PCR amplification generated a product of approximate molecular size 284 bp. 100 bp DNA marker was used as a molecular weight marker. The band size detected in all the Salmonella spp. isolates and S. typhimurium reference strain was consistent as analyzed by agarose gel electrophoresis. Of the 24 isolates, all isolates were confirmed to be Salmonella spp. genus level by invar gene based PCR. The results revealed that all the 24 suspected isolates were confirmed to be Salmonella spp. and were harboring the invAvirulence gene, indicating (100%) distribution of invA.