Vol. 11, Special Issue 10 (2022)

Author(s):
Shivani Mamane, N Jeyathilakan, BR Latha and TM A Senthilkumar

Abstract:
Visceral schistosomiasis caused by Schistosoma spindale is a neglected chronic wasting blood fluke illness of livestock wherein the adult worms are obligate parasite of blood vascular system. This chronic disease is accompanied with symptoms such as frequent diarrhoea with traces of blood and mucous, anemia, edema, substantial reduction in productivity and emaciation, often goes undiagnosed. The present study concentrated on analyzing the excretory secretary proteins of Schistosoma spindale by SDS-PAGE and also checking their immune reactivity using Western blot. Excretory Secretory proteins were prepared by incubating live adult worms collected from slaughter house. Several fractions of polypeptide bands were observed upon electrophoretic separation with approximate molecular weight of 14, 28, 57 and 66 kDa sizes. ES proteins obtained were probed by immunostaining with hyperimmune sera raised against them in rabbits. The Western blot analysis revealed presence of three immunodominant polypeptides of approximate molecular weight of 28, 57 and 66 kDa. The study showed immune reactive fractions of Schistosoma spindale ES proteins which can be further used in the diagnostic aids.