Vol. 11, Special Issue 10 (2022)

Author(s):
Chungada AS, Gahukar SJ, Akhare AA, Behere GT, Solanke AU and Patil SR

Abstract:
Genetic variability and fingerprint profiles of six citrus species were determined using different SCAR markers. In the present investigation, eleven different SCAR markers were validated for discriminating Galgal rootstock from other citrus species under study. The DNA amplification was carried out with eleven SCAR markers. The primer-1, primer-2, primer-3, primer-4, primer-5, primer-6 and primer-11 show the band size of 283 bp, 380 bp, 172 bp, 310 bp, 150 bp, 137 bp and 300 bp respectively. All these primers are promising primers for discrimination of Galgal from other species under study. Two SCAR primers viz, primer-1 and primer-6 showed 100% polymorphism followed by primer-2 and primer-3 showed 88% polymorphism with average polymorphism of all primers i.e. 75.90%. Total alleles per locus were 8.27, whereas the average no of monomorphic and polymorphic alleles was 1.63 and 6.54 respectively. The extent of polymorphic information content (PIC) values of eleven SCAR primers ranged from 0.39 to 0.86 with an average value of 0.63. The highest PIC value was observed in primer-1 (0.86), followed by primer-2 (0.76). The minimum PIC value was in primer-10 (0.39). All these primers are promising primers for discrimination of Galgal from other species under study. Out of these primers primer-1, primer-2, primer-6 and primer-11 are the best primers for discrimination of Galgal from Rangpur lime, Alemow, Jambhiri, Orange and Sweet Orange. The highest similarity was found between Jambhiri and Alemow with a correlation coefficient value of 0.48. The lowest similarity coefficient was observed between Orange and Galgal with a similarity coefficient value of 0.22. The cluster analysis revealed that Galgal rootstock is more diverse from other five species under study. These results will be very useful in testing the genetic purity of citrus at nursery stage.