Vol. 11, Special Issue 10 (2022)

Author(s):
Bithika Halder, Samar Sarkar, Samiran Bandapadhyay, Prasanta Kumar Sarkar, Amit Raj Gupta, Subhasish Batabyal and Kaushik Pal

Abstract:
Materials and Methods: In this case, both in-vitro and in-vivo experiment were performed. In-vitro experiment was carried out by following three methods i.e. agar well diffusion, plate diffusion and disc diffusion method. And in vivo experiment was started when the birds were 21 days of age. The birds were divided into three groups i.e. group-C served as control group, group-E treated with Enrofloxacin which act as standard control group and group-RS treated with test drug Rasa-sindoora, containing six birds in each group. The experiment (drug trial) was started on the day 22 and continued for next 28 days. On the day 0, 7, 14, 21 and 28 of experiment faecal sample and on day 0, 7, 14 and 28 of experiment blood sample were collected from each bird of three groups to examine the gastrointestinal gram-negative bacterial count and to evaluate the haemato-biochemical parameters i.e. Hb %, SGPT, SGOT, ALP, Urea and Creatinine. After 28 days of experiment, at the age of 50^th day, the birds were slaughtered to collect tissue sample from heart, lung, liver and kidney. Illeo-caecal junction tissue was also collected to examine the illeo-caecal coliform count. Results: In-vitro assessment showed no antibacterial efficacy but in in-vitro experiment fecal coliform count decreased significantly in RS treated group in comparison to control group. There were no significant (p<0.01) alteration in haemoglobin percentage, urea and creatinine level but serum SGPT, SGOT and ALP increased significantly in RS treated group. The illeo-caecal coliform count was decreased significantly in standard control and Rasa-sindoora treated groups in both dilutions 1:50 and 1:100 in comparison to normal control group. The result indicate that the test drug rasa-sindoora able to decrease the fecal and illeo-caecal coliform count but histopathological findings clearly showed the drug causes chronic toxicity in birds of RS treated group.