Vol. 11, Special Issue 10 (2022)

Author(s):
Shivashanker R, Nagaraja, Siddaling Swamy Hiremath, Shrikrishna Isloor, Shankrappa Bajantri and Yogesh

Abstract:
A study was conducted to ascertain the polymorphism of Bovine lactoferrin gene and its association with mastitis in HF crossbred and Deoni cattle. Milk and blood samples were collected from 152 HF crossbred and 92 Deoni cattle. Milk samples were screened for subclinical mastitis using three indirect tests viz. California Mastitis Test (CMT), Electrical conductivity and Somatic Cell Count (SCC). Depending on these test animals were grouped into affected and healthy. Genomic DNA was isolated by high salt method from blood samples, the quantity and quality of DNA was ascertained by spectrophotometer and 0.8 percent agarose gel electrophoresis. An intron 6 region of BLTF gene was amplified by PCR using published primer. The PCR-RFLP method using EcoRI enzyme revealed two genotypes AA and AB in both studied population. BB genotype was totally absent. Frequency of AA genotype 0.37, 0.71 and for AB genotype 0.71, 0.29 in HF crossbred and Deoni cattle respectively. The gene frequency was 0.69, 0.85 and 0.31, 0.15 for C and D in HF crossbred and Deoni cattle respectively. Chi-square (χ₂) analysis revealed that there is significant difference between these genotypes regarding mastitis incidence in Deoni cattle. AA genotyped animals were found to be less susceptible for mastitis, where as in HF crossbred there was no association was found.