Vol. 11, Special Issue 10 (2022)

Globally, infection cause by methicillin-resistant Staphylococcus aureus (MRSA) poses a severe threat to public health. One of the first-line medications for the treatment of MRSA and other resistant Gram-positive strains infections is still glycopeptide vancomycin. However, + isolates with complete resistance to vancomycin have emerged in recent years. In present study total 135 swab samples collected from clinically infected animals in Anand, Gujarat. Total 83 Staphylococcus isolates isolated from 135 samples and screened for vancomycin resistance genes and vancomycin resistance patterns by disc diffusion. Out of 83 staphylococci isolates 29 isolates showed resistance by disc diffusion method whereas all the isolates were found sensitive to vancomycin by E-test. During molecular detection of vancomycin resistance gene (vanA and vanB), only two isolates found positive for vanB gene. This isolate was found susceptible on disc diffusion as well as E-test suggest that this gene might not be expressed for the antagonistic effects against vancomycin.