Vol. 11, Special Issue 11 (2022)

Author(s):
BK Pati and Reena Mukherjee

Abstract:
Among the various mastitis pathogens, Staphylococcus aureus (S. aureus) is identified as chief etiological agent responsible for clinical, sub clinical and chronic mastitis and most challenging for the Veterinary clinician to treat such cases. In the present study 435 cows were screened for mastitis by California mastitis test and Somatic cell count from the Northern tropical region of India. On the basis of cultural and biochemical properties 61 isolates were presumptively identified as S. aureus. Further genotypically identified as S. aureus by Polymarase chain reaction (PCR) and 52 isolates were confirmed by species specific 16S rRNA and for thermonuclease gene (nuc). The present study might help to understand the distribution of prevalent S. aureus infection in the dairy farms and comparison between standard morphological tests and molecular characterization techniques.