Vol. 11, Special Issue 11 (2022)

Author(s):
HK Dalai, N Sahoo, S Meher, P Meher, SM Nayak, S Biswal and M Baguli

Abstract:
Canine vector-borne diseases constitute an important group of illnesses affecting dogs, that includes haemoparasites (e.g., Babesia vogeli, Leshmania infantum and Trypanosoma cruzi), bacteria (e.g., Anaplasma platys and Ehrlichia canis), and helminths (e.g., Dirofilaria immitis and Dipylidium caninum) that are transmitted by a diverse range of arthropod vectors. Canine vector-borne diseases are endemic throughout India. However, their identity to a species level remains anecdotal. The present study was undertaken to know the prevalence of canine vector borne diseases like Anaplasmosis, Borreliosis, Ehrlichiosis and Dirofilariosis through polymerase chain reaction and record the risk factors associated with prevalent vector-borne diseases in Koraput District of Odisha. Ninety six blood samples collected randomly from dogs were subjected to PCR for detection of antigen. Ninteen dogs were found positive for Ehrlichia when subjected to PCR. It was again subjected to PCR with species specific primers and detected Ehrlichia canis in all the 19 samples. All positive PCR products were sent to a Molecular laboratory for sequencing to confirm the validity of PCR amplifications. Sequences were compared with Gen Bank by BLAST analysis. It showed 88-93% similarity with many E. canis species in other geographical regions worldwide. The present study revealed prevalence of Ehrlichiosis in Koraput district. Therefore appropriate treatment and management practices should be adopted to reduce the occurrence of the disease.