Vol. 11, Special Issue 11 (2022)

Mycoplasmosis is one of the economically important diseases among small ruminants having direct impact on sheep and goat rearing farmers. An attempt was made to isolate and identify Mycoplasma from sheep and goats. A total of 121 samples (62 from sheep and 59 from goats) comprising of nasal discharges, milk, lung tissues and synovial fluids were subjected for isolation of Mycoplasma organisms in PPLO broth and PPLO agar. Inoculated samples were incubated at 37 °C for 24-48 hrs in CO₂ incubator with 5% CO₂, until turbidity and colour change was seen. Twenty-two samples produced characteristic fried egg micro colonies suggestive of Mycoplasma on PPLO agar. Mycoplasma isolates were further confirmed by PCR using Genus and M. arginini species specific primers. Two isolates were identified as M. arginini by PCR. Isolation of Mycoplasma as the gold standard test, along with PCR would give a rapid identification technique for these bacteria.