Vol. 11, Special Issue 12 (2022)

The aim of this study was to evaluate spermatozoan motility and the physiological level of acid phosphatase (ACP), alkaline phosphatase (ALT) in the luminal fluid collected from the three different epididymal segments from slaughtered Black Bengal bucks and the relationship between ACP and ALP level to sperm motility. The percentage of motile sperm was highest in the cauda segment (81.1 ± 1.23) followed by corpus (21.9 ± 0.84) and totally absent in caput region with significant difference (p<0.01) in between regions. Moreover, the correlation of spermatozoan motility between corpus and cauda was related (r = 0.812) which was highly significant (p<0.01). Concentration (U/L) of both ACP and ALP was highest in caudal segment of epididymis (6.95 ± 0.77 vs 7.97 ± 0.82) compare to corpus epididymis (6.14±0.43 vs 6.64±0.39) and caput epididymis (6.03 ± 0.35 vs 6.72 ± 0.46) respectively. Spermatozoan motility with ACP (r = 0.432) and ALP (r = 0.538) was highly positively correlated (p<0.01). The value of ACP and ALP was varied significantly (p<0.05) between caput and corpus, between caput and cauda (r = 0.513) and non-significantly between corpus and cauda (r = 0.305). Present investigation confirms that ACP, ALP and sperm motility, exhibited a good correlation with all the parameters studied, might be used as a simple analytical tool to monitor as one of the indicator for sperm maturation process in comparison with other conventional evaluations which require further analysis.