Vol. 12, Special Issue 7 (2023)

For buffalo bulls, the ejaculate having a sperm motility of 70% is only processed for sperm cryopreservation. But we can utilize the ejaculates which have lower motility by centrifugation. Therefore, in our study, we assessed the effect of single-layer centrifugation (SLC) and double-layer centrifugation (DLC) on before-freezing motility and quality of frozen semen. We selected 7 ejaculates of low sperm motility and divided them into three categories Cat-I without centrifugation (WC), Cat-II SLC, and Cat-III DLC. After that, we checked before freezing motility and cryopreserved them. After 24 hours of cryopreservation analyzed the post-thaw motility, sperm morphology, sperm plasma membrane integrity, and sperm acrosome integrity. On the basis of the results of these parameters DLC and SLC was better in semen quality than without centrifugation of lower motility ejaculates.