Vol. 12, Special Issue 7 (2023)

Author(s):
Pawar Purushotham, Kalyani Putty, Vishweshwar Kumar Ganji and Dhanalakshmi K

Abstract:
The canine parvovirus-2 (CPV-2) is a member of the Parvoviridae family and is responsible for severe and fatal viral enteritis in canines. It has been observed that several outbreaks of this virus have occurred due to the emergence of new variants, such as CPV-2a, CPV-2b, and CPV-2c. The objective of this study was to rapidly identify and isolate the CPV-2 in clinical cases using real-time PCR and growth in cell cultures. Out of the 100 rectal/fecal swab samples analyzed, 67 were positive for CPV-2 in Real-time PCR. In conventional PCR, 50 samples were typed as CPV-2b type whereas other 17 samples were typed as CPV-2a type. The isolates were recovered 57 samples in Crandell-rees canine kidney cells (CRFK) and 10 samples in Madin-darby canine kidney cells (MDCK). These isolates can serve as a basis for study of variations in CPV-2 and formulating vaccines with circulating variant types.