Vol. 12, Special Issue 7 (2023)

Author(s):
Diwakar, Akriti, AK Kataria and Sudesh Kumar

Abstract:
In this study, we employed PCR with specific primers targeting the cap5k and cap8k genes to conduct molecular characterization of capsule of 34 Staphylococcus aureus isolates originated from caprine clinical mastitis cases. The purpose was to identify the presence and distribution of these genes within the bacterial strains. The PCR results revealed that two isolates displayed amplify of 361 base pairs, indicating the presence of the cap5k gene. Additionally, 22 isolates exhibited amplify of 173 base pairs, indicating the presence of the cap8k gene. Furthermore, a subgroup of 10 isolates exhibited both types of amplify, confirming the simultaneous presence of both cap5k and cap8k genes. Consequently, the cap5k gene was found in 12 (35.29%) S. aureus and the cap8k gene was identified in 32 (94.11%) isolates. These findings provide valuable insights into the molecular composition and diversity of capsule among S. aureus isolates originating from caprine clinical mastitis, possibly aiding in advancing the comprehension of the pathogenesis and epidemiology of this caprine ailment.